Detection of mycotoxins produced by Fusarium species in Colombia

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dc.creatorClaudia Salazar-González
dc.creatorDavid Velásquez-Ortiz
dc.creatorEyder Gómez-López
dc.date2020
dc.date.accessioned2026-07-07T02:43:58Z
dc.descriptionThe genus Fusarium produces mycotoxins that are metabolites of low molecular weight that affect the quality of crops, and even more importantly, they affect the health of humans and animals. Among those that cause damage to health are tricho-thecenes, fumonisins, and zearalenones. The objective of this study was to quantify the mycotoxins produced by species of the genus Fusarium from a population of isolates obtained from different crops and locations in Colombia. From 206 isolates, only 14 amplified to regions associated with mycotoxins de-oxynivalenol (DON) belonging to the group of trichothecenes, fumonisin (FUM) and zearalenone (ZEA) using PCR. Each isolate with the presence of mycotoxins was conserved in potato dextrose agar (PDA) medium. Eight days after seeding in corn kernel medium, the samples were processed to perform the quantitative analysis of DON, ZEA and FUM using an ELISA kit based on enzyme-linked immunosorbent assays. The results show that mycotoxins were present in the evaluated isolates and their levels were above the standards regulated by Mercosur and the European Union. The use of immunosorbent assays using the ELISA technique becomes a useful tool to detect and quantify mycotoxins of species of the genus Fusarium that affect different crops in Colombia.
dc.formatapplication/pdf
dc.identifier0120-9965
dc.identifierhttps://www.redalyc.org/articulo.oa?id=180376041005
dc.identifierhttps://www.redalyc.org/journal/1803/180376041005/
dc.identifierhttps://www.redalyc.org/journal/1803/180376041005/html/
dc.identifierhttps://www.redalyc.org/journal/1803/180376041005/180376041005.epub
dc.identifierhttps://www.redalyc.org/journal/1803/180376041005/movil
dc.identifier10.15446/agron.colomb.v38n2.77176
dc.identifier.urihttp://hdl.handle.net/123456789/422157
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1803
dc.rightsAgronomía Colombiana
dc.sourceAgronomía Colombiana (Colombia) Num.2 Vol.38
dc.subjectAgrociencias
dc.titleDetection of mycotoxins produced by Fusarium species in Colombia
dc.typeartículo científico

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Agronomía Colombiana - Universidad Nacional de Colombia