Incorporation of Labeled Methionine as a Tissue Tracer in Broiler Chickens

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dc.creatorAC Stradiotti
dc.creatorJA Bendassolli
dc.creatorC Ducatti
dc.creatorJR Sartori
dc.creatorVC Pelícia
dc.creatorPC Araujo
dc.creatorMK Maruno
dc.creatorAC Pezzato
dc.date2016
dc.date.accessioned2026-07-07T03:24:50Z
dc.descriptionThe objective of this study was to evaluate the process of L-methionine incorporation in the blood plasma, liver, breast muscle, and abdominal fat of 35- to 59-d-old broiler chickens using the carbon stable isotope ( 12C and 13C) technique for the estimation of methionine requirements. In this experiment, 51 male broiler chickens orally received a solution of L-[13C1 ] methionine (92 atm % 13C) at 29 µmol/kg live weight/h for 6 h. Three birds were sacrificed for tissue collection at times 0 h (control), 0.5, 1, 2, 3, 4, 5, 6, 12, 24, 48, 72, 96, 120, 144, 168, and 336 h after the administration of the first dose. Tissue L-[13C1 ] methionine incorporation mass and percentage results were analyzed using Minitab 16 statistical software. Except for abdominal fat, tissue methionine levels gradually increased after the administration of the methionine solution. The calculated half-lives of methionine in the blood plasma, liver, and breast muscle were 2.52, 1.36, and 3.57 h, respectively, suggesting a greater rate of methionine incorporation in the liver, followed by blood plasma and breast muscle. The isotopic dilution showed that 2.81, 4.79, and 23.64% of the administered L-methionine were retained in the blood plasma, liver, and breast muscle, respectively. The methionine requirements of finisher broilers may be estimated using the carbon isotope technique, and approximately 3, 5, and 24% methionine is used for the synthesis of blood plasma, liver, and breast muscle, respectively, at the evaluated dose.
dc.formatapplication/pdf
dc.identifier1516-635X
dc.identifierhttps://www.redalyc.org/articulo.oa?id=179759360023
dc.identifier.urihttp://hdl.handle.net/123456789/433290
dc.languageen
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relationhttp://www.redalyc.org/revista.oa?id=1797
dc.rightsRevista Brasileira de Ciência Avícola
dc.sourceRevista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.18
dc.subjectAgrociencias
dc.titleIncorporation of Labeled Methionine as a Tissue Tracer in Broiler Chickens
dc.typeartículo científico

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Revista Brasileira de Ciência Avícola - Fundação APINCO de Ciência e Tecnologia Avícolas