Micropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant

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dc.creatorAmbajo, F.
dc.creatorMatheka, J.M.
dc.date2016-08-10
dc.date2016-09-09T12:21:09Z
dc.date2016-09-09T12:21:09Z
dc.date.accessioned2026-06-27T15:55:38Z
dc.descriptionAn efficient micropropagation protocol was developed for the medicinal plant Launaea cornuta using green house axillary buds as explants. The best sterility was obtained at 30% (v/v) local bleach (JIK). Maximum shoot induction rate was achieved when axillary buds were cultured on Murashige and Skoog (MS) Media supplemented with 0.5 mg/L of 6-benzylaminopurine (BAP) for 3 weeks. The highest number of shoot multiplication was obtained when induced shoots were culture on MS media supplemented with 0.5 mg/L BAP and 0.2 mg/L NAA for 30 days. The best rooting response with regard to average root length, rooting percentage and number of roots was achieved within 4 weeks of culture of excised shoots on MS media having 0.5 mg/L BAP. Regenerated plants were successfully acclimatized and about 80 to 90% of plantlets survived under ex vitro conditions. About 170 plants were produced from a single nodal bud of L. cornuta after 60 days. A reproducible protocol was established for in vitro propagation of L. cornuta, an important indigenous vegetable with high medicinal value.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/76989
dc.identifier.urihttp://hdl.handle.net/123456789/117656
dc.languageen
dc.publisherAcademic Journals
dc.rightsOpen Access
dc.sourceAmbajo, F. & Matheka, J.M. (2016). Micropropagation of Launaea cornuta-an important indigenous vegetable and medicinal plant. African Journal of Biotechnology, 15(32), 1726-1730.
dc.subjecttissue culture
dc.subjectmicropropagation
dc.subjectmedicinal plant
dc.titleMicropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)