INTRASPECIFIC VARIABILITY IN TETRAPLOID VARIETIES (4X) OF POTATO (Solanum tuberosum L.)
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Instituto Nacional de Ciencias Agrícolas
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Six intraspecific crosses, among three commercial varieties of potato -Desirée, Atlantic and Kondor- and four clones coming from the International Potato Center -CIP 23, 110, 114 and 115- were studied during two campaigns (1997/1998, 1998/1999), at the National Institute of Agricultural Sciences (INCA), by means of electrophoretic techniques, with the objective of determining their genetic variability and the possibility of their use in the potato breeding program. Two isozymatic systems, esterases and peroxidases were used. Eighteen samples were taken per each cross, along with the parents, from the first true leaf of the seedlings. A qualitative analysis was carried out for the presence or absence of bands and a quantitative analysis for the calculation of Jaccard¿s index of similarity. With the total values of the index of similarity for both systems, a test ¿t¿ was made to determine any significant differences among the crosses. The greatest variability was found inside each cross than among them, then the crosses Desirée x CIP 23, Desirée x CIP 115 and Desirée x CIP 110 stood out. The esterase system showed a higher polymorphism and detected a bigger variability than the peroxidase system.
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