New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust.

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dc.contributorDÊNIA PIRES DE ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; DEISY GUIMARÃES CARNEIRO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCA; DANÚBIA RODRIGUES ALVES, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA.
dc.creatorALMEIDA, D. P. de
dc.creatorCARNEIRO, D. G.
dc.creatorCAIXETA, E. T.
dc.creatorALVES, D. R.
dc.creatorZAMBOLIM, L.
dc.date2025-01-03T19:47:03Z
dc.date2025-01-03T19:47:03Z
dc.date2025-01-03
dc.date2024
dc.date.accessioned2026-07-01T00:27:26Z
dc.descriptionCoffee leaf rust, caused by Hemileia vastatrix, is the most important disease affecting Coffea arabica. The use of molecular markers through marker-assisted selection (MAS) is an effective approach to develop cultivars with multiple and durable resistance, which is economically and environmentally beneficial for this disease control. In this work, a new molecular marker based on allele-specific polymerase chain reaction (AS-PCR) associated with the quantitative trait locus (QTL) of the linkage group 5 (LG5), which confers resistance to races I, II and pathotype 001 of H. vastatrix, was developed. The CaRHv10-AS marker was validated in a segregating genetic mapping population of 247 F2 genotypes and demonstrated selection efficiency greater than 97% for genotypes with resistance to three races. Thus, this work provides a fast, robust, and affordable molecular marker for use in MAS, facilitating the development of coffee plants with multiple resistance and ensuring sustainable coffee production.
dc.identifierCrop Breeding and Applied Biotechnology, v. 24, n. 4, e498424410, 2024.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1171166
dc.identifierhttps://doi.org/10.1590/1984-70332024v24n4a47
dc.identifier.urihttp://hdl.handle.net/123456789/404492
dc.languageeng
dc.rightsopenAccess
dc.subjectHemileia Vastatrix
dc.subjectPlant breeding
dc.subjectSustainable agriculture
dc.subjectMarker-assisted selection
dc.titleNew and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust.
dc.typeArtigo de periódico

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