Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division

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dc.audienceInvestigador
dc.creatorOpalko, Hannah E.
dc.creatorMiller, Kristi E.
dc.creatorKim, Hyun Soo
dc.creatorVargas Garcia, Cesar Augusto
dc.creatorSingh, Abhyudai
dc.creatorKeogh, Michael Christopher
dc.creatorMoseley, James B.
dc.date2024-02-23T14:18:20Z
dc.date2024-02-23T14:18:20Z
dc.date2021
dc.date2021
dc.date.accessioned2026-06-27T04:41:35Z
dc.descriptionFission yeast cells prevent mitotic entry until a threshold cell surface area is reached. The protein kinase Cdr2 contributes to this size control system by forming multiprotein nodes that inhibit Wee1 at the medial cell cortex. Cdr2 node anchoring at the cell cortex is not fully understood. Through a genomic screen, we identified the conserved GTPase Arf6 as a component of Cdr2 signaling. Cells lacking Arf6 failed to divide at a threshold surface area and instead shifted to volume-based divisions at increased overall size. Arf6 stably localized to Cdr2 nodes in its GTP-bound but not GDP-bound state, and its guanine nucleotide exchange factor (GEF), Syt22, was required for both Arf6 node localization and proper size at division. In arf6<U+0394> mutants, Cdr2 nodes detached from the membrane and exhibited increased dynamics. These defects were enhanced when arf6<U+0394> was combined with other node mutants. Our work identifies a regulated anchor for Cdr2 nodes that is required for cells to sense surface area.
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dc.identifierhttps://doi.org/10.1083/jcb.202109152
dc.identifier0021-9525
dc.identifierhttp://hdl.handle.net/20.500.12324/38936
dc.identifier10.1083/jcb.202109152
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/35572
dc.languagespa
dc.publisherRockfeller University Press
dc.relationJournal of Cell Biology
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dc.rightsAttribution-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-sa/4.0/
dc.sourceJournal of Cell Biology; Vol. 221, Núm. 2 (2021):Journal of Cell Biology;p. 1 -16.
dc.subjectInvestigación agropecuaria - A50
dc.subjectGenética molecular
dc.subjectCiclo celular
dc.subjectDivisión celular
dc.subjectProcesos biológicos
dc.subjectTransversal
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_27577
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_37218
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_1413
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_330999
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/8394ab20-57b8-4370-a653-e21440ec643b/download
dc.titleArf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
dc.titleArf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
dc.typeArtículo científico

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