Genotyping SSR length variants by isothermal DNA amplification

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dc.creatorLee, David
dc.creatorIalicicco, Manuela
dc.creatorAkkinepalli, Harika
dc.creatorMorreale, Giacomo
dc.creatorLiu, Yan
dc.creatorLeung, Hei
dc.creatorScippa, Gabriella S.
dc.creatorGreenland, Andy
dc.creatorMackay, Ian
dc.date2012-09
dc.date2024-12-19T12:55:25Z
dc.date2024-12-19T12:55:25Z
dc.date.accessioned2026-06-27T04:15:00Z
dc.descriptionLoop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.
dc.identifierhttps://hdl.handle.net/10568/165746
dc.identifier.urihttp://hdl.handle.net/123456789/26539
dc.languageen
dc.publisherCanadian Science Publishing
dc.sourceLee, D., Ialicicco, M., Akkinepalli, H., Morreale, G., Liu, Y., Leung, H., Scippa, G.S., Greenland, A. and Mackay, I. 2012. Genotyping SSR length variants by isothermal DNA amplification. Genome, Volume 55 no. 09 p. 691-695
dc.subjectdna
dc.subjectalleles
dc.subjectdna amplification
dc.subjectdna sequencing
dc.subjectgenetic markers
dc.subjectgenotypes
dc.subjectmethods
dc.subjectmicrosatellites
dc.subjectnucleotide sequences
dc.subjectpolymerase chain reaction
dc.subjectsimple sequence repeats
dc.subjectvarieties
dc.titleGenotyping SSR length variants by isothermal DNA amplification
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)