Determination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.

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dc.contributorSILMARA ROSSANA BIANCHI, CNPS; Clarice Dias Britto do Amaral, UFPR; Caroline Santos da Silva, UFSCar; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.
dc.creatorBIANCHI, S. R.
dc.creatorAMARAL, C. D. B. do
dc.creatorSILVA, C. S. da
dc.creatorNOGUEIRA, A. R. de A.
dc.date2019-05-22T01:10:24Z
dc.date2019-05-22T01:10:24Z
dc.date2017-11-14
dc.date2017
dc.date2019-05-22T01:10:24Z
dc.date.accessioned2026-07-07T05:27:30Z
dc.descriptionDetermination of Se by inductively coupled plasma mass spectrometry (ICP-MS) can be difficult due to spectral interferences; however, these interferences can be eliminated by using collision-reaction interface (CRI) technology. A simple and fast procedure for determination of total Se concentration in bovine semen by ICP-MS after sample solubilization with diluted formic acid is here proposed. Formic acid caused an increase of up to 50% in selenium net signals and the maximum gain was obtained at 10% v v-1 formic acid solution. The optimized nebulizer gas flow rates were 1.05 and 0.95 L min-1, and radio frequency applied power were 1.3 and 1.4 kW without and with CRI, respectively. Microwave-assisted acid digestion was also implemented in the preparation of semen sample for comparison of results. No statistical differences between these sample preparation strategies at 95% confidence level (t-test) were observed for 76Se+ , 77Se+ , 78Se+ and 82Se+ without using CRI and 82Se+ with CRI.
dc.identifierJournal of the Brazilian Chemical Society, v. 28, n. 12, p. 2357-2364, 2017.
dc.identifier0103-5053
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1079670
dc.identifierhttp://dx.doi.org/10.21577/0103-5053.20170089
dc.identifier.urihttp://hdl.handle.net/123456789/491842
dc.languageeng
dc.rightsopenAccess
dc.subjectBovine semen
dc.subjectCattle fertility
dc.subjectselenium
dc.titleDetermination of selenium in bovine semen by ICP-MS using formic acid for sample preparation.
dc.typeArtigo de periódico

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