Polymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model

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dc.audienceInvestigador
dc.creatorCortés Patiño, Sandra
dc.creatorBonilla, Ruth Rebeca
dc.date2025-02-12T20:28:37Z
dc.date2025-02-12T20:28:37Z
dc.date2015
dc.date2015
dc.date.accessioned2026-06-27T04:36:58Z
dc.descriptionPlant growth promoting bacteria (PGPB) enhances the growth of their hosts and can protect them from biotic and abiotic stresses. Bacterial inoculants contain one or more of these beneficial strains in a carrier material, which must be able to maintain the viability of the cells during the time of storage, and also guarantee the biological activity of the strains once applied in the soil. These inoculants can be solid, liquid, gel or oil-based, depending on the characteristics of the strains and the shelf life expected by the producers. In this study, we used a method of accelerated degradation to select a polymer and a concentration to maintain cell stability of a liquid inoculant based on the strain C16 Azospirillum brasilense. A screening at 45°C was made to compare the protectant effect of five polymers on the viability of the strain (p/v): carrageenan (1.5%), sodium alginate (1%), trehalose (10 mM), polyvinylpyrrolidone (2%), glycerol (10 mM) and phosphate saline buffer as control. Carrageenan and sodium alginate showed significant differences in cell viability over the use of other polymers (P < 0.05). We evaluated cell viability with these two polymers at three concentrations and three different temperatures (4, 28 and 45°C) for 60 days and determined the bacterial degradation rates. Based on the Arrhenius thermodynamic model, we calculated the time required to reduce cell concentration in three log units, and observed that the protectant activity of each polymer and each concentration depends on the temperature of storage. Cell viability was best preserved in all treatments at 4°C. In general, alginate prolonged cell viability at 28°C, and carrageenan at 45°C. Alginate at 1% and carrageenan at 0.75% showed a stable behavior (superior to the control) in the three evaluated temperatures, so we conclude that they can be used for a formulation of a liquid inoculant based on the strain C16 of A. brasilense.
dc.descriptionCorporation of Agricultural Research - Corpoica
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dc.formatapplication/pdf
dc.identifierhttps://www.ajol.info/index.php/ajb/article/view/121404
dc.identifier1684-5315
dc.identifierhttp://hdl.handle.net/20.500.12324/40677
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/33151
dc.languageeng
dc.publisherAfrican Journals Online
dc.relationAfrican Journal of Biotechnology
dc.relation14
dc.relation33
dc.relation2547
dc.relation2553
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceAfrican Journal of Biotechnology; Vol. 14, Núm. 33 (2025): African Journal of Biotechnology ;p. 2547-2553.
dc.subjectInvestigación agropecuaria - A50
dc.subjectEnergía de activación
dc.subjectDegradación
dc.subjectMuerte celular
dc.subjectCinética
dc.subjectTransversal
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_2a4f12af
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_2159
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_49882
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_6ede19b4
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/374fae31-5ba9-4b52-9059-6ab26f7fdd59/download
dc.titlePolymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model
dc.titlePolymers selection for a liquid inoculant of Azospirillum brasilense based on the Arrhenius thermodynamic model
dc.typeArtículo científico

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