ANOTHER NITROGEN-FIXING MICROORGANISM IN SUGARCANE STALKS: Bacillus brevis?
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Instituto Nacional de Ciencias Agrícolas
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In order to identify a white colony-growing microorganism in an LGI medium, coming from sugarcane stalk apoplastic sap, some experiments were carried out in which ML-318 variety was used. Then, apoplastic sap was extracted and the microorganism of interest was isolated as well as some morphological, cultural and biochemical tests were carried out, which revealed 65 % probability that the microorganism of interest belongs to Bacillus brevis. Nitrogenase activity was measured by acetylene to ethylene reduction. Results also indicate interaction between Acetobacter diazotrophicus and the white colony-growing microorganism identified as Bacillus brevis.
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