Nontargeted Metabolomics (Reverse phase) - Analysis of Foods Standard Operating Procedure (SOP)

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dc.creatorOdenkirk, Melanie T
dc.date2025-03-26
dc.date2026-01-13T15:20:19Z
dc.date2026-01-13T15:20:19Z
dc.date.accessioned2026-06-27T13:34:02Z
dc.descriptionThis Standard Operating Procedure (SOP) describes a nontargeted metabolomics workflow for comprehensive small molecule profiling in foods using solid-phase extraction and reverse phase liquid chromatography-mass spectrometry. The protocol involves extracting lyophilized food with aqueous methanol, followed by protein precipitation and SPE cleanup to remove lipids and matrix interferents. Samples are spiked with internal retention time standards, dried, reconstituted, and analyzed by high-resolution MS, with reference samples as quality controls for comparative profiling across diverse food matrices. For unresolved issues, users are encouraged to seek additional assistance via ptfionboarding@gmail.com.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/179764
dc.identifier.urihttp://hdl.handle.net/123456789/63441
dc.languageen
dc.rightsOpen Access
dc.sourceOdenkirk, M.T. (2025) Nontargeted Metabolomics (Reverse phase) - Analysis of Foods Standard Operating Procedure (SOP). 10 p.
dc.subjectagrobiodiversity
dc.subjectfood composition
dc.subjectanalytical methods
dc.subjectlipids
dc.subjectlipid content
dc.titleNontargeted Metabolomics (Reverse phase) - Analysis of Foods Standard Operating Procedure (SOP)
dc.typeTraining Material

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