Characterization and genetic analysis of a light- and temperature-sensitive spotted-leaf mutant in rice

Vista sencilla de ítem
dc.creatorHuang, Qi-Na
dc.creatorShi, Yong-Feng
dc.creatorYang, Yang
dc.creatorFeng, Bao-Hua
dc.creatorWei, Yan-Lin
dc.creatorChen, Jie
dc.creatorBaraoidan, Marietta
dc.creatorLeung, Hei
dc.creatorWu, Jian-Li
dc.date2011-08
dc.date2024-12-19T12:55:36Z
dc.date2024-12-19T12:55:36Z
dc.date.accessioned2026-06-27T04:12:27Z
dc.descriptionA rice spotted‐leaf mutant was isolated from an ethane methyl sulfonate (EMS) ‐induced IR64 mutant bank. The mutant, designated as spl30 (spotted‐leaf30), displayed normal green leaf color under shade but exhibited red‐brown lesions under natural summer field conditions. Initiation of the lesions was induced by light and the symptom was enhanced at 33 °C relative to 26 °C. Histochemical staining did not show cell death around the red‐brown lesions. Chlorophyll contents in the mutant were significantly lower than those of the wild type while the ratio of chlorophyll a/b remained the same, indicating that spl30 was impaired in biosynthesis or degradation of chlorophyll. Disease reaction patterns of the mutant to Xanthomonas oryzae pv. oryzae were largely unchanged to most races tested except for a few strains. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively named spl30(t), which co‐segregated with RM15380 on chromosome 3, and was delimited to a 94 kb region between RM15380 and RM15383. Spl30(t) is likely a novel rice spotted‐leaf gene since no other similar genes have been identified near the chromosomal region. The genetic data and recombination populations provided in this study will enable further fine‐mapping and cloning of the gene.
dc.identifierhttps://hdl.handle.net/10568/165879
dc.identifier.urihttp://hdl.handle.net/123456789/25100
dc.languageen
dc.publisherWiley
dc.sourceHuang, Qi‐Na; Shi, Yong‐Feng; Yang, Yang; Feng, Bao‐Hua; Wei, Yan‐Lin; Chen, Jie; Baraoidan, Marietta; Leung, Hei and Wu, Jian‐Li. 2011. Characterization and genetic analysis of a light- and temperature-sensitive spotted-leaf mutant in rice. JIPB, Volume 53 no. 8 p. 671-681
dc.subjectchlorophyll
dc.subjectchromosome mapping
dc.subjectchromosomes
dc.subjectgenes
dc.subjectgenetic analysis
dc.subjectleaves
dc.subjectlight
dc.subjectmutants
dc.subjectmutations
dc.subjectpathogens
dc.subjectplant pathogenic bacteria
dc.subjectrecombination
dc.subjecttemperature
dc.subjectxanthomonas oryzae pv
dc.subjectoryzae
dc.titleCharacterization and genetic analysis of a light- and temperature-sensitive spotted-leaf mutant in rice
dc.typeJournal Article

Archivos

Colecciones

Instituto Internacional de Investigación del Arroz (IRRI)