Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics

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dc.creatorWesemael, Jelle van
dc.creatorHueber, Y.
dc.creatorKissel, E.
dc.creatorCampos, N.
dc.creatorSwennen, Rony L.
dc.creatorCarpentier, Sebastien C.
dc.date2018
dc.date2018-04-26T09:03:09Z
dc.date2018-04-26T09:03:09Z
dc.date.accessioned2026-06-27T13:49:45Z
dc.descriptionThe fate of doubled genes, from allopolyploid or autopolyploid origin, is controlled at multiple levels, resulting in the modern day cultivars. We studied the root growth of 3 different triploid banana cultivars under control and osmotic stress conditions. The root growth of the allopolyploid ABB cultivar was 42% higher under control and 61% higher under osmotic stress. By integrating transcriptomics and proteomics, we studied the gene expression of all 3 cultivars, resulting in 2,749 identified root proteins. 383 gene loci displayed genotype specific differential expression whereof 252 showed at least one Single Amino Acid Polymorphism (SAAP). In the ABB cultivar, allele expressions supposedly follow a 1/3 and 2/3 pattern for respectively the A and the B allele. Using transcriptome read alignment to assess the homeoallelic contribution we found that 63% of the allele specific genes deviated from this expectation. 32 gene loci even did not express the A allele. The identified ABB allele- specific proteins correlate well with the observed growth phenotype as they are enriched in energy related functions such as ATP metabolic processes, nicotinamide nucleotide metabolic processes, and glycolysis.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/92389
dc.identifier.urihttp://hdl.handle.net/123456789/67032
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourcevan Wesemael, J., Hueber, Y., Kissel, E., Campos, N., Swennen, R. & Carpentier, S. (2018). Homeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics. Scientific Reports, 8, 1353-1363.
dc.subjectbananas
dc.subjectplantains
dc.subjectcultivars
dc.subjectnicotinamide
dc.subjectmusa
dc.subjectgenotypes
dc.subjectallopolyploid
dc.subjectautopolyploid
dc.subjecttranscriptomics
dc.subjectproteomics
dc.titleHomeolog expression analysis in an allotriploid non-model crop via integration of transcriptomics and proteomics
dc.typeJournal Article

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