Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops

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dc.creatorZhang, A.
dc.creatorWang, M.
dc.creatorLi, Z.
dc.creatorPanis, B.
dc.creatorBettoni, J.C.
dc.creatorVollmer, R.
dc.creatorXu, L.
dc.creatorWang, Q.
dc.date2023-01-11
dc.date2023-03-29T17:54:24Z
dc.date2023-03-29T17:54:24Z
dc.date.accessioned2026-06-27T17:58:01Z
dc.descriptionRoot and tuber crops (RTCs) are the second-most important carbohydrate commodity after cereals. Many species of the RTCs are vegetatively propagated, making their shoot tips the preferred material to be conserved for future uses. Shoot tip cryopreservation provides an important tool to support the long-term conservation of plant genetic resources. Over the past four decades, significant efforts have been undertaken to move shoot tip cryopreservation of RTCs from research projects to full-scale implementation in cryobanks. This comprehensive review focuses on the history of cryopreservation protocols developed in RTCs. The encapsulation and vitrification solution-based cryopreservation techniques followed by ultra-rapid freezing and thawing have been highly successful. Additionally, different strategies for improving the cryotolerance of shoot tips have been introduced to further increase post-cryopreservation recovery. Finally, the research conducted to explain the mechanism underlying cryoprotection and differential cryotolerance including the use of histological studies are highlighted.
dc.identifierhttps://hdl.handle.net/10568/129802
dc.identifier.urihttp://hdl.handle.net/123456789/150908
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceZhang, A.; Wang, M.; Li, Z.; Panis, B.; Bettoni, J. C.; Vollmer, R.; Xu, L.; Wang, Q. 2023. Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops. Agronomy. ISSN 2073-4395. 13(1). 219. 37 p.
dc.subjectcassava
dc.subjectcryopreservation
dc.subjectpotatoes
dc.subjectroot crops
dc.subjecttubers
dc.subjectsweet potatoes
dc.subjecttaro
dc.titleOvercoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops
dc.typeJournal Article

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