Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.

Vista sencilla de ítem
dc.audienceInvestigador
dc.creatorUribe, L.
dc.creatorBeltrán Acosta, C.
dc.creatorToloza Moreno, D.
dc.creatorSuárez, M.
dc.creatorLópez, M.
dc.creatorRodríguez, T.
dc.creatorBraissant, O.
dc.creatorBravo, D.
dc.date2025-02-13T13:37:08Z
dc.date2025-02-13T13:37:08Z
dc.date2021
dc.date2021
dc.date.accessioned2026-06-27T05:21:24Z
dc.descriptionScopulariopsis fungi genus has 112 recognized species in Index-Fungorum (http://www.indexfungorum.org/Names/Names.asp). Some species are classified as a human opportunistic FFHP (Jagielski et al., 2016). These species are able to produces toxins or carry allergens, which cause health hazards (Woudenberg et al. 2017). In environmental sciences, a new species of S. gossypii was categorized an opportunistic pathogen causing leaf interveinal chlorosis and vascular browning of cotton plants (Li et al. 2017). It has been isolated from a wide variety of substrates, containing both hyaline and somewhat pigmented filamentous fungi that normally are saprotrophs (Sandoval-Denis et al. 2013). Control of the pathogen Scopulariopsis sp. is conducted by chemical antifungals such as sertaconazole, terbinafine and tioconazole (Carrillo-Muñoz et al. 2004), however with high costs and low results in environmental conditions. Despite the presence of different Scopulariopsis sp. species facilitating vascular wilt, biological control as a strategy against this fungus is not common. A non-conventional technique that has been used recently in biological control of microorganisms within agro-ecosystems is isothermal microcalorimetry (IMC) (Yao et al. 2007). It is a sensitive method for real-time monitoring and dynamic analysis of chemical, physical and biological processes (Braissant et al. 2010) used to measure metabolic activity of relevant environmental microorganisms (Bravo et al., 2011). This research aims to determine the use of IMC to assess the antagonic activity of Trichoderma. asperellum (Th034 and Th406), the metabolic interaction between Th034, Th406, and Bacillus amyloliquefaciens (Bs006) against Scopulariopsis sp. (Sc002) either independently or in consortium. Using the kinetical parameter data obtained after fitting the Richards equation of growth, an Anova test was performed to compare the variance between the antagonic populations and the target population of Scopulariopsis sp. Using the maximum heat values (Qmax in J.h-1), the fractional inhibitory concentration index of Scopulariopsis sp. (FIC) was calculated as shown in a previous study (Fälcao et al., 2014). The FIC index was above the treshold for antagonic activity using T. asperellum Th406. Using a standardized medium developed in this study, this research demonstrates that Scopulariopsis sp., is inhibited due the metabolic activity of T. asperellum (Th406) measured by IMC with a FIC of 4.88. Thus, the strain Th406 is suggested as a promising microbial agent against Scopulariopsis sp. Sc002.
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.identifierchrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.kalorimetrietage.ptb.de/fileadmin/documents/kalorimetrietage/24Kaltage_2021/Posterabstracts/Uribe_Isothermal_microcalorimetry.pdf
dc.identifierhttp://hdl.handle.net/20.500.12324/40681
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/53972
dc.languageeng
dc.publisherCorporación colombiana de investigación agropecuaria - AGROSAVIA
dc.relation1
dc.relation2
dc.relationBraissant, O., Wirz, D., Göpfert, B., & Daniels, A. U. (2010). Use of isothermal microcalorimetry to monitor microbial activities.FEMS Microbiol Lett, 303(1), 1-8. Bravo, D., Braissant, O., Solokhina, A., Clerc, M., Daniels, A.U., Verrecchia, E. and Junier, P.
dc.relation(2011), Use of an isothermal microcalorimetry assay to characterize microbial oxalotrophic activity. FEMS Microbiol Ecol, 78: 266-274. https://doi.org/10.1111/j.1574-6941.2011.01158.x Carrillo-Muñoz, A. J., Guglietta, A., Palacín, C., Casals, J., Del Valle, O., Guardià, C., Rodríguez,
dc.relationV. and Quindós, G. (2004). In vitro antifungal activity of sertaconazole compared with nine other drugs against 250 clinical isolates of dermatophytes and Scopulariopsis brevicaulis. Chemotherapy, 50(6), 308-313.
dc.relationCuenca-Estrella, M., Gomez-Lopez, A., Mellado, E., Buitrago, M. J., Monzón, A., & Rodriguez- Tudela, J. L. (2003). Scopulariopsis brevicaulis, a fungal pathogen resistant to broad-spectrum antifungal agents. Antimicrob Agents Chemother, 47(7), 2339-2341.
dc.relationFalcäo, L., Silva‐Werneck, J., Vilarinho, B., da Silva, J., Pomella, A. and Marcellino, L. (2014), Antimicrobial and plant growth‐promoting properties of the cacao endophyte Bacillus subtilis ALB629. J Appl Microbiol, 116: 1584-1592. https://doi.org/10.1111/jam.12485
dc.relationJagielski, T., Sandoval-Denis, M., Yu, J., Yao, L., Bakuła, Z., Kalita, J., Skóra, M., Krzysciak, P., Hoog, G, Guarro, J. and Gené, J. (2016). Molecular taxonomy of Scopulariopsis-like fungi with description of new clinical and environmental species. Fungal Biol, 120(4), 586-602.
dc.relationLi, X. L., Ojaghian, M. R., Zhang, J. Z., & Zhu, S. J. (2017). A new species of Scopulariopsis and its synergistic effect on pathogenicity of Verticillium dahliae on cotton plants. Microbiol Res, 201, 12-20.
dc.relationSandoval-Denis, M., Sutton, D. A., Fothergill, A. W., Cano-Lira, J., Gené, J., Decock, C. A., ... & Guarro, J. (2013). Scopulariopsis, a poorly known opportunistic fungus: spectrum of species in clinical samples and in vitro responses to antifungal drugs. J Clinical Microbiol,51(12), 3937-3943.
dc.relationWoudenberg, J. H. C., Meijer, M., Houbraken, J. and Samson, R. A. (2017). Scopulariopsis and scopulariopsis-like species from indoor environments. Studies Mycol, 88, 1-35.
dc.relationYao, J., Xu, C., Wang, F., Tian, L., Wang, Y., Chen, H., Yong, Z., M F. Martin., Choi, E., Maskow, T. (2007). An in vitro microcalorimetric method for studying the toxic effect of cadmium on microbial activity of an agricultural soil. Ecotoxicology, 16(7), 503-509.
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectInvestigación agropecuaria - A50
dc.subjectScopulariopsis
dc.subjectTrichoderma
dc.subjectCalorimetría
dc.subjectTransversal
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_24703
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_15814
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_28276
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/3ce3817d-8313-4c32-946c-cb48cdc324e8/download
dc.titleIsothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
dc.titlePoster: Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
dc.typeArtículo de divulgación

Archivos

Colecciones

Tecnológico