MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene

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dc.creatorHe, Mingliang
dc.creatorZhang, Weibo
dc.creatorWang, Shan
dc.creatorGe, Ling
dc.creatorCao, Xiukai
dc.creatorWang, Shanhe
dc.creatorYuan, Zehu
dc.creatorLv, Xiaoyang
dc.creatorGetachew, Tesfaye
dc.creatorMwacharo, Joram
dc.creatorHaile, Aynalem
dc.creatorSun, Wei
dc.date2023-01-03T21:53:35Z
dc.date2023-01-03T21:53:35Z
dc.date.accessioned2026-06-27T14:24:45Z
dc.descriptionMicroRNA (miRNA) is of great importance to muscle growth and development, including the regulation of the proliferation and differentiation of skeletal muscle satellite cells (SMSCs). In our research group’s previous study, we found that miR-181a is differentially expressed in the longissimus dorsi muscle of Hu sheep at different stages. We speculated that miR-181a may participate in the growth and development process of Hu sheep. To understand the mechanism of miR-181a regulating the growth and development of Hu sheep skeletal muscle, we extracted skeletal muscle satellite cells from the longissimus dorsi muscle of 3-month-old Hu sheep fetuses and performed a series of experiments. Our results showed that miR-181a suppressed SMSCs’ proliferation using QRT-PCR, Western blot, CCK-8, EDU, and Flow cytometry cycle tests. In addition, QRT-PCR, Western blot, and immunofluorescence indicated that miR-181a facilitated the differentiation of SMSCs. Then, we used dual-luciferase reporter gene detection, QRT-PCR, and Western blot to find that the Yes1-related transcription regulator (YAP1) is the target gene of miR-181a. Our study supplies a research basis for understanding the regulation mechanism of miR-181a on the growth of Hu sheep skeletal muscle.
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dc.identifierhttps://hdl.handle.net/10568/126521
dc.identifier.urihttp://hdl.handle.net/123456789/78926
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceMingliang He, Weibo Zhang, Shan Wang, Ling Ge, Xiukai Cao, Shanhe Wang, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Joram Mwacharo, Aynalem Haile, Wei Sun. (16/3/2022). MicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene. Genes, 13 (3).
dc.subjectgoal 1 no poverty
dc.subjectsheep
dc.subjectdifferentiation
dc.subjecthu sheep
dc.subjectpoverty reduction, livelihoods and jobs
dc.subjectmir-181a
dc.subjectyap1
dc.subjectproliferation
dc.subjectsatellite cell
dc.titleMicroRNA-181a Regulates the Proliferation and Differentiation of Hu Sheep Skeletal Muscle Satellite Cells and Targets the YAP1 Gene
dc.typeJournal Article

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Centro Internacional para la Investigación Agrícola en las Áreas Secas (ICARDA)