A Mad7 system for genetic engineering of filamentous fungi.

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dc.contributorKATHERINA GARCIA VANEGAS, UNIVERSITY OF DENAMARK; JAKOB KRÆMMER HAAR RENDSVIG, UNIVERSITY OF DENMARK; ZOFIA DOROTA JARCZYNSKA, UNIVERSITY OF DENMARK; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ABEL PETER VAN ESCH, UNIVERSITY OF DENAMARK; MARTÍ MORERA-GÓMEZ, UNIVERSITY OF DENAMARK; FABIANO JARES CONTESINI, UNIVERSITY OF DENAMARK; UFFE HASBRO MORTENSEN, UNIVERSITY OF DENAMARK.
dc.creatorVANEGAS K. G.
dc.creatorRENDSVIG, J. K. H.
dc.creatorJARCZYSKA, Z. D.
dc.creatorCÔRTES, M. V. de C. B.
dc.creatorVAN ESCH, A. P.
dc.creatorMORERA-GÓMEZ, M.
dc.creatorCONTESINI, F. J.
dc.creatorMORTENSEN, U. H.
dc.date2023-02-02T16:01:20Z
dc.date2023-02-02T16:01:20Z
dc.date2023-02-02
dc.date2023
dc.date.accessioned2026-07-01T00:08:06Z
dc.descriptionThe introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories.
dc.identifierJournal of Fungi, v. 9, n. 1, 16, 2023.
dc.identifier2309-608X
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1151460
dc.identifierhttps://doi.org/10.3390/jof9010016
dc.identifier.urihttp://hdl.handle.net/123456789/398088
dc.languageeng
dc.rightsopenAccess
dc.subjectFilamentous fungi
dc.subjectCRISPR
dc.subjectMad7
dc.subjectFungal strain engineering
dc.subjectFungo
dc.subjectAspergillus
dc.subjectGenetic engineering
dc.subjectFungi
dc.titleA Mad7 system for genetic engineering of filamentous fungi.
dc.typeArtigo de periódico

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