Searching for nucleotide binding sites of resistance genes in wild Arachis germplasm.
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In this work, we used a PCR based approach to search for conserved regions in R genes in wild accessions of Arachis spp., especially the N-terminal nucleotide binding site (NBS). The products of PCR using this approach consisted of complex mixtures containing NBS regions, all of about 500bp.
Palabras clave
Gene resistente, Nucleotide binding sites, Genética Vegetal, Arachis