COMPARATIVE APPLICATION OF RFLP AND AFLP TO MONITORING GENETIC DIVERSITY AMONG A GROUP OF SUGARCANE VARIETIES AND THEIR RELATIONSHIP TO FAMILY PERFORMANCE
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Instituto Nacional de Ciencias Agrícolas
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Five sugarcane varieties and their hybrids were evaluated for brix, plant height and stalk weight components on seedling and first clonal stages. Correlations among RFLP (DR), AFLP (DA) and genealogical (DG) parent distances, the observed family means and five predictors of family performance were calculated. High and statistically significant correlations (r=0.88- 0.99*) between observed family means and midparent and highparent heterosis were obtained for all characters and plant stages. Cluster analysis groupings on parent distances differed. The cophenetic value of UPGMA cluster analysis increased when employing molecular distance estimates calculated, considering one of each pseudoallele variant with both types of markers (RFLP, AFLP). The presence of pseudoallelism did not influence the predictive value of parental distances for family means or heterosis. These results confirmed previous reports in different crops on the limited predictive value of molecular-based parent distance estimates when markers employed are not selected for their genetic linkage to loci controlling traits studied. Present results evidenced the practical value of BLUP method to estimate brix and stalk diameter family means, considering as effects the seedling stages and the parental pairwise genetic distances. These estimates attained high correlations values (r=0.81- 0.97*) with observed family means, although slightly lower than those obtained by LS estimates and the traditional family predictors based on progeny evaluation, such as the general combining ability and midparental heterosis. Better results could be expected increasing the number of markers and selecting them for their linkage relations and magnitude of their contribution to phenotype of target characters.
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