Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

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dc.creatorGonzález Amao, M.T.
dc.creatorPanta, A.
dc.creatorRoca, W.
dc.creatorEscobar Pérez, Roosevelt H.
dc.creatorEngelmann, F.
dc.date2007-11-29
dc.date2014-09-24T08:41:58Z
dc.date2014-09-24T08:41:58Z
dc.date.accessioned2026-06-27T14:40:25Z
dc.descriptionShoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks.
dc.identifierhttps://hdl.handle.net/10568/43329
dc.identifier.urihttp://hdl.handle.net/123456789/85069
dc.languageen
dc.publisherSpringer
dc.rightsLimited Access
dc.subjectcryopreservation
dc.subjectsomatic embryogenesis
dc.subjectcriopreservación
dc.subjectembriogénesis somática
dc.titleDevelopment and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops
dc.typeJournal Article

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Centro Internacional de Agricultura Tropical (CIAT)