Application of Doehlert experimental design in the optimization of experimental variables for the Pseudozyma sp. (CCMB 306) and Pseudozyma sp. (CCMB 300) cell lysis

Amanda  Reges de SENA; Gildomar   Lima VALASQUES JÚNIOR; Ingara Keisle   São Paulo BARRETTO; Sandra Aparecida  ASSIS

Application of Doehlert experimental design in the optimization of experimental variables for the Pseudozyma sp. (CCMB 306) and Pseudozyma sp. (CCMB 300) cell lysis

Autores

Amanda Reges de SENA

Gildomar Lima VALASQUES JÚNIOR

Ingara Keisle São Paulo BARRETTO

Sandra Aparecida ASSIS

Editor

Sociedade Brasileira de Ciência e Tecnologia de Alimentos

Descripción

This study aimed to verify the influence of pH and temperature on the lysis of yeast using experimental design. In this study, the enzymatic extract containing β-1,3-glucanase and chitinase, obtained from the micro-organism Moniliophthora perniciosa, was used. The experiment showed that the best conditions for lysis of Pseudozyma sp. (CCMB 306) and Pseudozyma sp. (CCMB 300) by lytic enzyme were pH 4.9 at 37 °C and pH 3.9 at 26.7 °C, respectively. The lytic enzyme may be used for obtaining various biotechnology products from yeast.