UTILIZACIÓN DE UN ENSAYO DE DILUCIÓN EN MICROPLATOS PARA MEDIR LA ACTIVIDAD ANTIFÚNGICA DE SUSTANCIAS CONTRA Mycosphaerella fijiensis, MORELET.

dc.creatorJorge Enrique Peláez Montoya
dc.creatorLuz Estella Vásquez David
dc.creatorThais Judith Díaz Brito
dc.creatorDarío Antonio Castañeda Sánchez
dc.creatorEsperanza Rodríguez Beltrán
dc.creatorRafael Eduardo Arango Isaza
dc.date2006
dc.date.accessioned2026-07-07T03:29:27Z
dc.descriptionBlack Sigatoka, caused by the fungus Mycosphaerella fijiensis is the most important disease in banana plantations. The fungus is controlled mainly by fungicide applications with an annual cost of about 350 million dollars in Latin and Central America. Due to the appearance of resistant strains and to the economical and environmental impact caused by the extensive use of fungicides, accurate methods are necessary for monitoring the fungal sensitivity to these agents. In this paper we describe the standarization of a method based on microplate dilutions that measures IC50 of different antifungal compounds against single ascospore cultures of M. fijiensis. The method was used to measure the sensitivity of 30 strains collected from different regions in Colombia against Propiconazol, Benomyl and Azoxystrobin. Used at a larger scale, this method could be useful to monitor M. fijiensis sensitivity against fungicides and to search for new compounds with activity against the fungus.
dc.formatapplication/pdf
dc.identifier0304-2847
dc.identifierhttps://www.redalyc.org/articulo.oa?id=179914075002
dc.identifier.urihttp://hdl.handle.net/123456789/435158
dc.languagees
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1799
dc.rightsRevista Facultad Nacional de Agronomía - Medellín
dc.sourceRevista Facultad Nacional de Agronomía - Medellín (Colombia) Num.2 Vol.59
dc.subjectAgrociencias
dc.titleUTILIZACIÓN DE UN ENSAYO DE DILUCIÓN EN MICROPLATOS PARA MEDIR LA ACTIVIDAD ANTIFÚNGICA DE SUSTANCIAS CONTRA Mycosphaerella fijiensis, MORELET.
dc.typeartículo científico

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