Comunicación corta . LA KINETINA RIBÓSIDO COMO ESTIMULADOR DE LA GERMINACIÓN In Vitro DE ESPORAS DE Glomus clarum
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Instituto Nacional de Ciencias Agrícolas
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Nowadays the in vitro mycorrhization of plants is a challenge in agricultural biotechnology and it depends of the germination potential of arbuscular mycorrhizal fungal (AMF) propagules, specially the spores, their colonization abilities and the media and systems of culture. The aim of this work was to evaluate the effect of two concentrations (0,05 mg L -1 y 0,07 mg L -1 ) of the auxin AIA and the cytochinin kinetin riboside on in vitro germination and germinative tube length of G. clarum spores in E medium (modified MS). E and MSR media were used as controls. All culture media had influence on both variables performance. The concentration of kinetin riboside of 0,07 mg L -1 had a positive effect on germination percentage, reaching values of 100 % in that medium after 10 days of incubation. Those values were statistically similar to those founded in MSR medium. However, the highest values of germinative tube length were obtained in MSR medium and they were 45 % higher to those measured in E medium combined with kinetin riboside (0,07 mg L -1 ). The AIA concentrations used had an inhibitory effect on spore germination and also on the germinative tubes growth
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