Callus Induction and Shoot Production in Capsicum Chinense (POD Type Scotch Bonnet)
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Various expiants excised from greenhouse-grown and in vitro-germinaled seedlings of Capsicum chinense were inoculated onto MS medium supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) or BA and indolacetic acid (IAA) treatments, to allow callus induction and shoot formation, respectively. All cultures were incubated at 28° C with a 16-hour photoperiod (3000 lux). All 2,4-D / BA treatments induced callus on stem, hypocotyls, and leaf expiants. The 0.8 mg/L 2,4-D and 1.5 mg/L BA, 2.0 mg/L 2,4-D and 3.0 mg/L Β A and 3.2 mg/1 2,4-D and 1.5 mg/'L BA treatments gave the best results. The calli produced were of compact, friable or mixed types. Shoot tip and nodal expiants produced shoots on all BA/IAA treatments and the control; bud burst ranged from 40% to 100%. IAA 1 mg/L and BA 8 mg/L gave 100% budburst and IAA 1 mg/L and Β A lmg/1 gave the greatest shoot height. No shoots were produced directly on leaf, hypocotyls, or seed leaf expiants. The response of expiants derived from greenhouse and that of those of in vitro origin were compared.