Enzymatic hydrolysis of cassava stalks pretreated with the alkaline method
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Universidad Nacional de Colombia
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This study was developed with the aim of evaluating the pH,enzymatic complex load and temperature effects on the sac-charification of pretreated cassava stalks (CS) using the re-sponse surface methodology (RSM). The factor levels evaluatedwere temperature 35 - 40°C, pH 4.0 - 5.0 and dose of enzymaticcomplex Accellerase 1500™2.9 - 14.5 FPU/g of substrate. Thereducing sugar (RS) response was used. The pH was controlledthrough the use of hydrochloric acid and sodium hydroxidesolutions and the system was shaken orbitally at 120 rpm witha solids loading of 10% w/v. The fitted model showed that theoptimal operating conditions were: pH 4.0, 38°C and enzymedose of 14.5 FPU/g substrate, reaching a sugar concentrationof 18.4 g L-1.
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