Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae).
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Bands of double-stranded RNA (dsRNA) were detected in three out of twelve isolates of Paecilomyces fumosoroseus. Identity of these bands was confirmed by RNAse, DNAse and S1 nuclease treatments. The cure of dsRNA for one isolate (P92) was successfully carried out for a single conidium subculture. Isogenic strains, with or without dsRNA, were submitted to virulence tests against the whitefly Bemicia tabaci strain B. In contrast to findings for some phytopathogenic fungi, these dsRNA fragments did not cause hypovirulence in P. fumosoroseus.
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Fungo entomopatogenico, Inseto praga, Paecilomyces fumosoroseus, Bemicia tabaci, Brasil, Entomogenous fungi, Pest insects, Controle Biológico, biological control, Brazil