The role of organic solvent amount in the lipase-catalyzed biodiesel production
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
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This research note addresses the role of organic solvent amount in the production of fatty acid ethyl esters from soybean oil. N-hexane was chosen as solvent and two commercial immobilized lipases as catalysts, Novozym 435 and l ipozyme I m . The reactions were conducted in 6 hours, varying the solvent to oil ratio from zero to 50 (v/wt) and adopting adopting for Novozym 435: 65 o C, enzyme concentration (E, wt%) = 5, oil to ethanol molar ratio (R) = 1:10, water addition (H, wt%) = 0, and for l ipozyme I m : 35 o C, E = 5 wt%, R = 1:3, H = 10 wt%. For l ipozyme I m , an increase in solvent amount is shown to lead to an enhancement of reaction conversion, while a negligible effect was found for Novozym 435. When using 30 m l of solvent the reaction conversions were 88% for l ipozyme I m and 15% for Novozym 435.
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