Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.

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dc.contributorELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG.
dc.creatorDORNELES, E. M. S.
dc.creatorSANTANA, J. A.
dc.creatorRIBEIRO, D.
dc.creatorDORELLA, F. A.
dc.creatorGUIMARAES, A. S.
dc.creatorMOAWAD, M. S.
dc.creatorSELIM, S. A.
dc.creatorGARALDI, A. L. M.
dc.creatorMIYOSHI, A.
dc.creatorRIBEIRO, M. G.
dc.creatorGOUVEIA, A. M. G.
dc.creatorAZEVEDO, V.
dc.creatorHEINEMANN, M. B.
dc.creatorLAGE, A. P.
dc.date2015-04-14T11:11:11Z
dc.date2015-04-14T11:11:11Z
dc.date2015-04-14
dc.date2014
dc.date2015-04-14T11:11:11Z
dc.date.accessioned2026-07-07T03:46:27Z
dc.descriptionThe aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
dc.identifierPlos One, v. 9, n. 6, e98758, 2014.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504
dc.identifierhttps://doi.org/10.1371/journal.pone.0098758
dc.identifier.urihttp://hdl.handle.net/123456789/442526
dc.languageeng
dc.rightsopenAccess
dc.subjectEnterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
dc.titleEvaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
dc.typeArtigo de periódico

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