Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies

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dc.creatorBishop, Richard P.
dc.creatorSohanpal, B.K.
dc.creatorSpooner, P.R.
dc.creatorDolan, T.T.
dc.creatorAllsopp, B.A.
dc.creatorMorzaria, S.P.
dc.date1993-07
dc.date2013-06-11T09:23:34Z
dc.date2013-06-11T09:23:34Z
dc.date.accessioned2026-06-27T17:06:42Z
dc.descriptionA total of 21Theileria parvastocks from 6 countries were characterized usingT. parvarepetitive and ribosomal DNA probes, aPlasmodium bergheitelomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots ofEcoRI-digestedT. parvaDNA revealed 20 different restriction fragment patterns among DNA samples isolated from infections initiated using 16 parasite stocks. The panel of anti-schizont MAbs defined 8 different profiles among schizont-infected lymphoblastoid cell-cultures infected with the same 16T. parvastocks. Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A clonedT. parvasmall subunit ribosomal RNA (SSUrRNA) gene probe separated 17T. parvastocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized toEcoRI-digestedT. parvaDNA. When hybridized toPvuII-digested DNA from 8T. parvastocks, the ribosomal probe identified 4 groups with similar restriction fragment patterns. A synthetic oligonucleotide derived from aP. bergheitelomeric sequence hybridized to 7 or 8 size-polymorphic restriction fragments in theEcoRI-digested DNA of mostT. parvastocks. The telomeric and ribosomal probes defined the same 4 groups among 8T. parvastocks as assessed by similarities in restriction fragment patterns. Based on the comparison of repetitive DNA sequences from theT. parvaUganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of theT. parvaUganda stock from that of 4 otherT. parvastocks on a positive/negative basis.
dc.identifierhttps://hdl.handle.net/10568/29440
dc.identifier.urihttp://hdl.handle.net/123456789/142511
dc.languageen
dc.publisherCambridge University Press
dc.rightsLimited Access
dc.sourceParasitology;107: 19-31
dc.subjecttheileria parva
dc.subjectmonoclonal antibodies
dc.subjectdna
dc.subjectanimal diseases
dc.titleDetection of polymorphisms among Theileria parva stocks using repetitive, telomeric ribosomal DNA probes and anti-schizont monoclonal antibodies
dc.typeJournal Article

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