Vigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019

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dc.audienceInvestigador
dc.coverageColombia
dc.creatorSaavedra, Sandra Yamile
dc.creatorBernal, Johan Fabian
dc.creatorMontilla Escudero, Efrain
dc.creatorTorres, German
dc.creatorRodríguez, Mabel Karina
dc.creatorHidalgo, Andrea Melissa
dc.creatorOvalle, María Victoria
dc.creatorRivera, Sandra
dc.creatorPerez Gutierrez, Enrique
dc.creatorDuarte, Carolina
dc.date2024-07-26T20:17:40Z
dc.date2024-07-26T20:17:40Z
dc.date2020
dc.date2020
dc.date.accessioned2026-06-27T04:28:14Z
dc.descriptionObjetivo. Describir las características epidemiológicas, fenotípicas y genéticas de aislamientos clínicos portadores de optrA identificados en la vigilancia de resistencia antimicrobiana por el laboratorio del Instituto Nacional de Salud de Colombia. Métodos. Entre octubre de 2014 y febrero 2019, se recibieron 25 aislamientos de Enterococcus spp. resistentes al linezolid. La identificación y sensibilidad antimicrobiana se determinó con Vitek 2 y la concentración inhibitoria mínima (CIM) al linezolid se estableció con E-test. El gen optrA se detectó mediante PCR. La diversidad genética de aislamientos positivos para optrA se analizó con Diversilab®. Se seleccionaron seis aislamientos para llevar a cabo la secuenciación del genoma completo. Resultados. Se confirmó el gen optrA en 23/25 aislamientos de E. faecalis de siete departamentos de Colombia. Los aislamientos presentaron una CIM al linezolid entre 8 y >256μg/mL. La tipificación por Diversilab® indicó una amplia variabilidad genética. Todos los aislamientos analizados mediante secuenciación del genoma completo, presentaron genes de resistencia fexA, ermB, lsaA, tet(M), tet(L) y dfrG además de optrA y fueron negativos para otros mecanismos de resistencia al linezolid. Se identificaron tres secuencias tipos y tres variantes de optrA: ST16 (optrA-2), ST476 (optrA-5) y ST618 (optrA-6). El entorno genético de los aislamientos optrA-2 (ST16) presentó el segmento impB, fex, optrA, asociado a plásmido, mientras que en dos aislamientos (optrA-6 y optrA-5) se encontró el elemento cromosómico transferible Tn6674-like. Conclusión. Los aislamientos clínicos positivos para optrA presentan una alta diversidad genética, con diferentes clones y variantes de optrA relacionados con dos tipos de estructuras y diferentes elementos genéticos móviles.
dc.descriptionRed PulseNet para América Latina y el Caribe (OPS/OMS).
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dc.identifierhttps://iris.paho.org/handle/10665.2/52679
dc.identifier1680 5348
dc.identifierhttp://hdl.handle.net/20.500.12324/39670
dc.identifierhttps://doi.org/10.26633/RPSP.2020.104
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/28653
dc.languagespa
dc.publisherPan American Health Organization
dc.relationRevista Panamericana De Salud Publica
dc.relation44
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceRevista Panamericana De Salud Publica; Vol. 44 (2020): Revista Panamericana De Salud Publica;p. 1-10
dc.subjectInvestigación agropecuaria - A50
dc.subjectStreptococcus faecalis
dc.subjectFarmacología
dc.subjectGanadería y especies menores
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_16605
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_5760
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/c9f535ce-a158-40b7-8afa-b824ef2a8a19/download
dc.titleVigilancia nacional de aislamientos clínicos de Enterococcus faecalis resistentes al linezolid portadores del gen optrA en Colombia, 2014-2019
dc.titleNational surveillance of clinical isolates of Enterococcus faecalis resistant to linezolid carrying the optrA gene in Colombia, 2014-2019
dc.typeArtículo científico

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