Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã

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dc.creatorLuciene de Oliveira Ribeiro
dc.creatorLuciano Vilela Paiva
dc.creatorMarlúcia Souza Pádua
dc.creatorBreno Régis Santos
dc.creatorEduardo Alves
dc.creatorVanessa Cristina Stein
dc.date2012
dc.date.accessioned2026-07-07T03:53:46Z
dc.descriptionThis work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 - transparent watery callus, Type 2 - yellow callus with small clusters, Type 3 - yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were isodiametric, which is a characteristic of embryogenic cells. The TEM analysis showed that Type 1 callus cells had thin walls, a large number of small vacuoles and dispersed cytoplasm. The Type 2 callus cells showed dense cytoplasm, large vacuoles and a large amount of mitochondria. The Type 3 callus cells had thick and intercellular spaces. Thus, the Type 2 callus cells had characteristics consistent with embryogenic callus cells.
dc.formatapplication/pdf
dc.identifier1679-9275
dc.identifierhttps://www.redalyc.org/articulo.oa?id=303025342009
dc.identifier.urihttp://hdl.handle.net/123456789/446520
dc.languageen
dc.publisherUniversidade Estadual de Maringá
dc.relationhttp://www.redalyc.org/revista.oa?id=3030
dc.rightsActa Scientiarum. Agronomy
dc.sourceActa Scientiarum. Agronomy (Brasil) Num.4 Vol.34
dc.subjectAgrociencias
dc.titleMorphological and ultrastructural analysis of various types of banana callus, cv. Prata anã
dc.typeartículo científico

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Acta Scientiarum. Agronomy - Universidade Estadual de Maringá