Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa

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dc.creatorTomaszewska, Paulina
dc.creatorPellny, Till K.
dc.creatorHernández, Luis Miguel
dc.creatorMitchell, Rowan A. C.
dc.creatorCastiblanco, Valheria
dc.creatorVega, Jose J. de
dc.creatorSchwarzacher, Trude
dc.creatorHeslop-Harrison, J.S.
dc.date2021-06-23
dc.date2021-11-09T15:21:53Z
dc.date2021-11-09T15:21:53Z
dc.date.accessioned2026-06-27T13:26:34Z
dc.descriptionUrochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands. We aimed to develop an optimized approach to determine ploidy of germplasm collection of this tropical forage grass group using dried leaf material, including approaches to collect, dry and preserve plant samples for flow cytometry analysis. Our methods enable robust identification of ploidy levels (coefficient of variation of G0/G1 peaks, CV, typically <5%). Ploidy of some 348 forage grass accessions (ploidy range from 2x to 9x), from international genetic resource collections, showing variation in basic chromosome numbers and reproduction modes (apomixis and sexual), were determined using our defined standard protocol. Two major Urochloa agamic complexes are used in the current breeding programs at CIAT and EMBRAPA: the ’brizantha’ and ’humidicola’ agamic complexes are variable, with multiple ploidy levels. Some U. brizantha accessions have odd level of ploidy (5x), and the relative differences in fluorescence values of the peak positions between adjacent cytotypes is reduced, thus more precise examination of this species is required. Ploidy measurement of U. humidicola revealed aneuploidy.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/115921
dc.identifier.urihttp://hdl.handle.net/123456789/59525
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceTomaszewska, P.; Pellny, T.K.; Hernández, L.M.; Mitchell, R.A.C.; Castiblanco, V.; de Vega, J.J.; Schwarzacher, T.; Heslop-Harrison, P. (2021) Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa s. l. Genes. 12(7):957. ISSN: 2073-4425
dc.subjectbrachiaria
dc.subjectpanicum
dc.subjectfeed grasses
dc.subjectploidy
dc.subjectflow cytometry
dc.subjectapomixis
dc.subjectdry matter
dc.subjectleguminosas forrajeras
dc.subjectploidía
dc.subjectcitometría de flujo (células)
dc.subjectextracto seco
dc.titleFlow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa
dc.typeJournal Article

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