In vitro regeneration in cole crops
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The experiment was conducted to optimize a suitable protocol for in vitro regeneration in cole crops. Callus initiation
was excellent in the variety Early Tropical. Highest percentage of callus proliferation was observed in Early Tropical
(75.0%) followed by Tangail Special Pauslali (55.0%) and the lowest in Tara (40.0% ). Maximum callus proliferation
(68.5%) was observed in MS + 3.0 mgL-1 BAP + 0.1 mgL-1 2,4-D + 2.0 mgL-1 AgNO3 . Callus proliferation was lowest
(40.0%) in MS + 2.5 mgL-1 BAP + 0.1 mgL-1 2,4-D + 2.0 mgL-1 AgNO3. MS medium supplemented with 3.0 mgL-1
BAP + 1.0 mgL-1 2,4-D + 2.0 mgL-1 AgNO3 was the best for shoot initiation & plantlet regeneration. The highest
number of shoots per vial was 7.20 and the lowest number of shoots per vial was 4.40. Among the concentration MS
+ 3.0 mgL-1 BAP + 0.1 mgL-1 2,4-D + 2.0 mgL-1 AgNO3 showed the highest performance of shoots per vial. The
variety Tangail Special Pauslali was the best for root initiation.