A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes

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dc.creatorKyoko, H.
dc.creatorMasakazu, H.
dc.creatorRyo, N.
dc.creatorYoshimasa, T.
dc.creatorJung-Yeon, K.
dc.creatorNoboru, I.
dc.creatorNene, Vishvanath M.
dc.creatorChihiro, S.
dc.date2010-11
dc.date2010-06-14T08:26:59Z
dc.date2010-06-14T08:26:59Z
dc.date.accessioned2026-06-27T17:19:39Z
dc.descriptionTheileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the transcription factor NF-κB is activated in schizont–infected cells, which makes the T. parva-transformed cells resistant to apoptosis. However, the mechanism by which the parasite triggers the activation of NF-κB remains enigmatic. In the present study, we biochemically characterized a novel protein, which we termed TpSCOP, which is expressed in the schizont stage of T. parva. TpSCOP was shown to interact with F-actin in vitro. Expression of TpSCOP in a murine lymphocytic cell line resulted in the activation of NF-κB signaling pathways, leading to apoptosis resistance. The activation of MAPKs, including ERK and JNK, were also detected. Furthermore, the introduction of TpSCOP into T. parva-infected cells also enhanced the activation of NF-κB. This is the first report to demonstrate that parasite-derived molecule has the ability to activate the host NF-κB pathway. Based on these results, TpSCOP likely plays an important role in apoptosis inhibition during Theileria infection.
dc.identifierhttps://hdl.handle.net/10568/1921
dc.identifier.urihttp://hdl.handle.net/123456789/148979
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceKyoko Hayashida; Masakazu Hattori; Ryo Nakao; Yoshimasa Tanaka; Jung-Yeon Kim; Noboru Inoue; Nene, V.; Chihiro Sugimoto. 2010. A schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes. Molecular and Biochemical Parasitology
dc.subjecttheileria parva
dc.subjectcattle
dc.titleA schizont-derived protein, TpSCOP, is involved in the activation of NF-κB in Theileria parva-infected lymphocytes
dc.typeJournal Article

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