Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.

Vista sencilla de ítem
dc.contributorTaís Jobard S. Macedo; Jamile Maiara S. Santos; Maria Éllida S. Bezerra; Vanúzia G. Menezes; Bruna B. Gouveia; Lara Mariane R. Barbosa; Thae Lane Barbosa G. Lins; Alane Pains O. Monte; Ricássio S. Barberino; André M. Batista; Vanessa Raquel P. Barros; Aurea Wischral; MARIO ADRIANO A. QUEIROZ; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; Maria Helena T. Matos.
dc.creatorMACEDO, T. J. S.
dc.creatorSANTOS, J. M. S.
dc.creatorBEZERRA, M. E. S.
dc.creatorMENEZES, V. G.
dc.creatorGOUVEIA, B. B.
dc.creatorBARBOSA, L. M. R.
dc.creatorLINS, T. L. B. G
dc.creatorMONTE, A. P. O.
dc.creatorBARBERINO, R. S.
dc.creatorBATISTA, A. M.
dc.creatorBARROS, V. R. P.
dc.creatorWISCHRAL, A.
dc.creatorQUEIROZ, M. A. A.
dc.creatorARAUJO, G. G. L. de
dc.creatorMATOS, M. H. T.
dc.date2019-11-14T18:28:56Z
dc.date2019-11-14T18:28:56Z
dc.date2019-11-14
dc.date2019
dc.date2019-11-18T11:11:11Z
dc.date.accessioned2026-07-07T05:55:04Z
dc.descriptionThe aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.
dc.identifierMolecular and Cellular Endocrinology, v. 495, sept. 2019.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397
dc.identifier10.1016/j.mce.2019.110506
dc.identifier.urihttp://hdl.handle.net/123456789/502552
dc.languageeng
dc.rightsopenAccess
dc.subjectImuno-histoquímica
dc.subjectMaturação de oócitos
dc.subjectDesenvolvimento folicular
dc.subjectLeptina
dc.subjectImunoexpressão de proteínas receptoras
dc.subjectOvino
dc.subjectOvário
dc.titleImmunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
dc.typeArtigo de periódico

Archivos

Colecciones

Embrapa Semi-Árido (CPATSA)