Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.

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dc.contributorVIVIAN ANGÉLICO PEREIRA ALFRADIQUE, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorCAROLINA CERQUEIRA SARMENTO OLIVARES, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorJOANNA MARIA GONÇALVES DE SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorJEFERSON FERREIRA DA FONSECA, CNPC
dc.contributorRIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorMÁRIO FELIPE ALVAREZ BALARO, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorHELENA FABIANA REIS DE ALMEIDA SARAIVA, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorLUANA RANGEL CÔRTES, UNIVERSIDADE FEDERAL FLUMINENSE
dc.contributorFELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE.
dc.creatorALFRADIQUE, V. A. P.
dc.creatorOLIVARES, C. C. S.
dc.creatorSOUZA-FABJAN, J. M. G. de
dc.creatorFONSECA, J. F. da
dc.creatorBATISTA, R. I. T. P.
dc.creatorBALARO, M. F. A.
dc.creatorSARAIVA, H. F. R. de A.
dc.creatorCÔRTES, L. R.
dc.creatorBRANDÃO, F. Z.
dc.date2020-01-23T00:34:17Z
dc.date2020-01-23T00:34:17Z
dc.date2020-01-22
dc.date2019
dc.date2020-01-23T11:11:11Z
dc.date.accessioned2026-07-01T00:21:48Z
dc.descriptionBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique.
dc.identifierActa Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1119218
dc.identifier10.22456/1679-9216.98317
dc.identifier.urihttp://hdl.handle.net/123456789/402612
dc.languageeng
dc.rightsopenAccess
dc.subjectBillygoats
dc.subjectCTC
dc.subjectCaprino
dc.subjectOvino
dc.subjectBovino
dc.subjectBode
dc.subjectCarneiro
dc.subjectReprodução Animal
dc.subjectSêmen
dc.subjectHeparina
dc.subjectEspermatozóide
dc.subjectSheep
dc.subjectGoats
dc.subjectCattle
dc.subjectRams
dc.subjectBulls
dc.subjectAnimal reproduction
dc.subjectSperm capacitation
dc.subjectAssisted reproductive technologies
dc.subjectHeparin
dc.titleMini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.
dc.typeArtigo de periódico

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