Diversity in the regulatory region of genes in the SH3 locus.
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RATIONALE: The SH3 locus is implicated in plant defense against the leaf rust disease caused by the fungi Hemileia vastatrix, which reduces coffee plant production. A cluster of genes coding for NB-LRR proteins is allocated to this locus. Investigating the regulatory region of' these genes can be worthy in knowledge and economically. METHODS: The cis-acting elements (cis-els, conserved motifs for the interaction with transcription machinery) in the promoters (2000 pb up-stream translation start) of 20 genes allocated to paralogous SH3 loci in four coffea spp. genotypes were identified and counted (www.dna.affrc.go.jp/place). Data were analyzed using Sigma Plot. RESULTS: Cis-els more frequently identified are depicted in Fig. 1 (in average, 3.5 times/promoter for SEF4MOTIFGM7S up to 14.1 times for CAATBOXl), Genes diverged for the frequency of cis-els. At least one gene/genotype was found in the highest cis-el frequency class (11.46 < N3, A2, Cl, B8 > 11.9 copies of each cis-el), probably displaying complex patters of induction. Genotypes did not diverge. CONCLUSIONS & PERSPECTIVES: Information presented here are going to be correlated with the transcription level of different genes.
ASIC. S2-P-20.
ASIC. S2-P-20.
Palabras clave
Genética Vegetal, Genotype, Coffea, Species diversity