Soybean B-Glucosidase immobilisated on chitosan beads and its application in soy drink increase the Aglycones.

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dc.contributorLUCIANA CARVALHO GRADE, UEL; AMANDA ALEIXO MOREIRA, UEL; GENI DA SILVA VAREA, UEL; JOSÉ MARCOS GONTIJO MANDARINO, CNPSO; JOSEMEYRE BONIFÁCIO DA SILVA, UEL; ELZA IOUKO IDA, UEL; MARA LÚCIA LUIZ RIBEIRO, UEL.
dc.creatorGRADE, L. C.
dc.creatorMOREIRA, A. A.
dc.creatorVAREA, G. da S.
dc.creatorMANDARINO, J. M. G.
dc.creatorSILVA, J. B. da
dc.creatorIDA, E. I.
dc.creatorRIBEIRO, M. L. L.
dc.date2014-09-17T07:37:21Z
dc.date2014-09-17T07:37:21Z
dc.date2014-09-03
dc.date2014
dc.date2014-09-17T07:37:21Z
dc.date.accessioned2026-07-07T11:52:17Z
dc.descriptionThe objective of this study was to investigate the immobilisation efficiency of soybean β-glucosidase (181.6 U/mL; 23.8 mg protein/mL) on activated chitosan beads. Central Composite Rotational Design (CCDR) 2 was used and the application of immobilised enzyme in commercial soy drink was evaluated. The activation of chitosan beads was achieved with established 2.5% glutaraldehyde, pH 7.5, 8 h incubation time (6 h with agitation and 2 h without agitation) at 37ºC. The highest immobilisation efficiency (%) of soybean β-glucosidase on chitosan beads obtained was 37.74 U/mL and 18.84 mg protein/4 chitosan beads at pH 7.5 and 20 h coupling time of enzyme-matrix (7 h with agitation and 13 h without agitation) at 4ºC. The immobilised enzyme incubated at 50ºC, pH 5.5 resulted in 24% increase in the aglycones content in commercial soy drink after 60 min.
dc.identifierBrazilian Archives of Biology and Technology, Curitiba, v. 57, n. 5, p. 766-773, Sept./Oct. 2014.
dc.identifier1516-8913
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/994090
dc.identifierhttp://dx.doi.org/10.1590/S1516-8913201402331
dc.identifier.urihttp://hdl.handle.net/123456789/515735
dc.languageeng
dc.rightsopenAccess
dc.subjectSoja
dc.subjectSoybeans
dc.titleSoybean B-Glucosidase immobilisated on chitosan beads and its application in soy drink increase the Aglycones.
dc.typeArtigo de periódico

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