Further investigations on the polysaccharides obtained from the stems and caps Crocybe titans.
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The giant mushroom Macrocybe titans have drawn the attention on account of their food potential. A chemical analysis was carried out on its stems and caps, in order to determine their carbohydrate structures. Each portion was extracted with CHCI3-MeOH and then MeOH-H20 to remove low-molecular weight compounds. Each resulting residue was submitted, successively, to aqueous extraction (x 6) and 2% aq. KOH (x 6) at 100ºC, and the extracted polysaccharides, which were recovered by ethanol precipitation (WP, KP, WS, and KS fractions), were dialyzed against tap water for 24 h, and freeze-dried. The polysaccharide fractions were then submitted to purification procedures by freeze-thawing to give insoluble (|- WP, I-KP, I-WS, and I-KS fractions) and water-soluble fractions (S-WP, S- WS, S- KP, and S-KS fractions). According to the GC-MS analysis of derived alditol acetates, all the insoluble-water fractions had glucose as its main monosaccharides, while those of soluble fractions were Gal, Gle, Fuc, and Man.
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Macrocybe titans, Cogumelo, Polissacarídeo