Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.

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dc.contributorLUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
dc.creatorOTERO, R.
dc.creatorHERNÁNDEZ, D.
dc.creatorCAMARGO, L. S. de A.
dc.date2018-12-26T23:42:43Z
dc.date2018-12-26T23:42:43Z
dc.date2018-12-26
dc.date2018
dc.date2019-01-04T11:11:11Z
dc.date.accessioned2026-07-07T03:47:18Z
dc.descriptionAbstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.
dc.format9 p.
dc.identifierIndian Journal of Science and Technology, v. 11, n. 25, 2018.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1102530
dc.identifier10.17485/ijst%2F2018%2Fv11i25%2F128251
dc.identifier.urihttp://hdl.handle.net/123456789/442903
dc.languageeng
dc.rightsopenAccess
dc.subjectEmbryonic Development
dc.subjectEpigenetic Modification
dc.subjectNuclear Transfer
dc.titleEffect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.
dc.typeArtigo de periódico

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