Identification of candidate genes regulating drought tolerance in pearl millet

Vista sencilla de ítem
dc.creatorChakraborty, Animikha
dc.creatorViswanath, Aswini
dc.creatorMalipatil, Renuka
dc.creatorSemalaiyappan, Janani
dc.creatorShah, Priya
dc.creatorRonanki, Swarna
dc.creatorRathoe, Abhishek
dc.creatorSingh, Sumer Pal
dc.creatorGovindaraj, Mahalingam
dc.creatorTonapi, Vilas A.
dc.creatorThirunavukkarasu, Nepolean
dc.date2022
dc.date2025-07-14T18:35:06Z
dc.date2025-07-14T18:35:06Z
dc.date.accessioned2026-06-27T15:06:10Z
dc.descriptionPearl millet is an important crop of the arid and semi-arid ecologies to sustain food and fodder production. The greater tolerance to drought stress attracts us to examine its cellular and molecular mechanisms via functional genomics approaches to augment the grain yield. Here, we studied the drought response of 48 inbreds representing four different maturity groups at the flowering stage. A set of 74 drought-responsive genes were separated into five major phylogenic groups belonging to eight functional groups, namely ABA signaling, hormone signaling, ion and osmotic homeostasis, TF-mediated regulation, molecular adaptation, signal transduction, physiological adaptation, detoxification, which were comprehensively studied. Among the conserved motifs of the drought-responsive genes, the protein kinases and MYB domain proteins were the most conserved ones. Comparative in-silico analysis of the drought genes across millet crops showed foxtail millet had most orthologs with pearl millet. Of 698 haplotypes identified across millet crops, MyC2 and Myb4 had maximum haplotypes. The protein–protein interaction network identified ABI2, P5CS, CDPK, DREB, MYB, and CYP707A3 as major hub genes. The expression assay showed the presence of common as well as unique drought-responsive genes across maturity groups. Drought tolerant genotypes in respective maturity groups were identified from the expression pattern of genes. Among several gene families, ABA signaling, TFs, and signaling proteins were the prospective contributors to drought tolerance across maturity groups. The functionally validated genes could be used as promising candidates in backcross breeding, genomic selection, and gene-editing schemes in pearl millet and other millet crops to increase the yield in drought-prone arid and semi-arid ecologies.
dc.identifierhttps://hdl.handle.net/10568/175630
dc.identifier.urihttp://hdl.handle.net/123456789/94598
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceChakraborty, Animikha; Viswanath, Aswini; Malipatil, Renuka; Semalaiyappan, Janani; Shah, Priya; Ronanki, Swarna; Rathoe, Abhishek; Singh, Sumer Pal; Govindaraj, Mahalingam; Tonapi, Vilas A.; and Thirunavukkarasu, Nepolean. 2022. Identification of candidate genes regulating drought tolerance in pearl millet. International Journal of Molecular Sciences 23(13): 6907. https://doi.org/10.3390/ijms2313690713
dc.subjectpearl millet
dc.subjectdrought resistance
dc.subjectfunctional genomics
dc.subjectcandidate genes
dc.subjectagriculture
dc.titleIdentification of candidate genes regulating drought tolerance in pearl millet
dc.typeJournal Article

Archivos

Colecciones

Instituto Internacional de Investigación sobre Políticas Alimentarias (IFPRI)