Erinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.

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dc.contributorWILLIAM SIHLER, Cenargen; MARCIO MARTINELLO SANCHES, CNPGC; MURILO FAZOLIN, CPAF-AC; JOELMA LIMA VIDAL ESTRELA; MARLINDA LOBO DE SOUZA, Cenargen.
dc.creatorSIHLER, W.
dc.creatorSANCHES, M. M.
dc.creatorFAZOLIN, M.
dc.creatorESTRELA, J. L. V.
dc.creatorSOUZA, M. L. de
dc.date2024-02-19T17:32:17Z
dc.date2024-02-19T17:32:17Z
dc.date2024-02-19
dc.date2024
dc.date.accessioned2026-07-07T11:54:34Z
dc.descriptionThe hornworm (Erinnyis ello) is an important pest of cassava and rubber tree with high capacity of migration and can be controlled with biological control agents such as baculoviruses. Baculoviruses are rod-shaped enveloped viruses with circular double-stranded DNA genomes infecting insects of the order Lepidoptera. Prior to large-scale use of certain baculovirus, it is essential to assess its genetic and morphological information. This work aimed to characterize the baculovirus isolate occurring in Erinnyis ello populations at small farms in Cruzeiro do Sul, Acre State, Brazil and to develop parameters for field management using this isolate. Viral particles were purified from infected larvae through ultracentrifugation in sucrose gradient. After treatment and negative staining with uranyl acetate 2%, viral particles were visualized using transmission electron microscope. The virus with ovicylindrical occlusion body presenting only one virion per envelope and size less than 0.5 µm was identified as a member of the genus Betabaculovirus. Viral DNA was extracted through phenol/chloroform cycles, digested with different restriction enzymes and separated with agarose gel electrophoresis. The restriction enzyme patterns obtained with the enzymes Bam HI and Hind III revealed seven and three DNA fragments, respectively; cleavage with Pst I, generated eight molar fragments and three submolar fragments and Eco RI digestion resulted in 21 fragments, with some submolar bands. Comparison of the DNA restriction pattern from ErelGV isolated in Acre with that described for ErelGV isolated from SC State showed high similarity. This isolate named as ErelGV-Acre presented potential for control of E. ello populations.
dc.identifierTechnology in Agronomy, v. 4, e001, 2024.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1162096
dc.identifierhttps://doi.org/10.48130/tia-0023-0019
dc.identifier.urihttp://hdl.handle.net/123456789/517169
dc.languageeng
dc.rightsopenAccess
dc.subjectRubber tree
dc.subjectCaterpillar
dc.subjectHornworm
dc.subjectPlagas de plantas
dc.subjectYuca
dc.subjectÁrbol de goma
dc.subjectControl biológico
dc.subjectComunidades rurales
dc.subjectCultivo a pequeña escala
dc.subjectCruzeiro do Sul (AC)
dc.subjectAcre
dc.subjectAmazônia Ocidental
dc.subjectWestern Amazon
dc.subjectAmazonia Occidental
dc.subjectPraga de Planta
dc.subjectMandioca
dc.subjectManihot Esculenta
dc.subjectSeringueira
dc.subjectHevea Brasiliensis
dc.subjectLagarta
dc.subjectErinnyis Ello
dc.subjectControle Biológico
dc.subjectBaculovirus
dc.subjectPequeno Produtor
dc.subjectPropriedade Rural
dc.subjectPlant pests
dc.subjectCassava
dc.subjectLepidoptera
dc.subjectBiological control
dc.subjectBaculoviridae
dc.subjectDsDNA viruses
dc.subjectRural communities
dc.subjectSmall-scale farming
dc.titleErinnyis ello granulovirus for cassava hornworm control in the Acre State of Brazil.
dc.typeArtigo de periódico

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