Characterization of phylogenetically distinct temperate phages from Kenyan Mammaliicoccus sciuri

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dc.creatorCherbuin, J.D.R.
dc.creatorLlodra, J.
dc.creatorBorcard, L.
dc.creatorKaessmeyer, S.
dc.creatorRamette, A.
dc.creatorFernandez, J.E.
dc.creatorWagner, T.M.
dc.creatorTorres-Puig, S.
dc.creatorKuhnert, P.
dc.creatorTurner, D.
dc.creatorLabroussaa, F.
dc.creatorJores, Joerg
dc.date2025-12
dc.date2026-03-04T06:45:03Z
dc.date2026-03-04T06:45:03Z
dc.date.accessioned2026-06-27T16:48:39Z
dc.descriptionBackground: Temperate bacteriophages are widespread in bacterial genomes and can play significant roles in bacterial evolution and pathogenicity. Despite their importance, they remain poorly characterized in nonclinical <i>Staphylococcaceae</i>, particularly <i>Mammaliicoccus sciuri</i>. Materials and Methods: We analyzed 26 <i>M. sciuri</i> strains isolated from the nasal cavities of East African dogs and camels. Prophages were induced using mitomycin C, and isolated phages were characterized by whole-genome sequencing, phylogenetic analysis, electron microscopy imaging, and host-range determination. Results: Eight novel siphoviruses were isolated. Phylogenomic analysis revealed two new families, each comprising two genera. Notably, phages from one of these families (with genomes >130 kbp) exhibit a broad host range, while the other family is related to previously described phages implicated in horizontal gene transfer. Conclusion: Our findings reveal unexpected diversity of temperate phages in <i>M. sciuri</i>, expanding current knowledge of phage distribution in animal-associated opportunistic pathogens.
dc.identifierhttps://hdl.handle.net/10568/181909
dc.identifier.urihttp://hdl.handle.net/123456789/133812
dc.languageen
dc.publisherSAGE Publications
dc.rightsOpen Access
dc.sourceCherbuin, J.D.R., Llodra, J., Borcard, L., Kaessmeyer, S., Ramette, A., Fernandez, J.E., Wagner, T.M., Torres-Puig, S., Kuhnert, P., Turner, D., Labroussaa, F. and Jores, J. 2025. Characterization of phylogenetically distinct temperate phages from Kenyan <i>Mammaliicoccus sciuri</i>. PHAGE: Therapy, Applications, and Research 6 (4): 259–271.
dc.subjectbacteriophages
dc.subjectcamels
dc.subjectdogs
dc.titleCharacterization of phylogenetically distinct temperate phages from Kenyan Mammaliicoccus sciuri
dc.typeJournal Article

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