Freedom to operate analysis, design and evaluation of expression cassettes that confer tolerance to glyphosate

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dc.creatorJenny Jiménez-Barreto
dc.creatorJulián Mora-Oberlaender
dc.creatorAlejandro Chaparro-Giraldo
dc.date2020
dc.date.accessioned2026-07-07T02:43:19Z
dc.descriptionTolerance to the herbicide glyphosate is the most extended feature in commercial transgenic events released worldwide and it is an example of the successful use of genetic modification to improve weed control in crops. Glyphosate-tolerant genotypes have been developed by multinational corporations using patent-protected technologies. For some of these events, all associated patents have expired and, therefore, have become a good target for the development of herbicide-tolerant agbiogen erics by national research institutions, using local crop varieties. As a first step in this process, we present the design (in silico) of three expression cassettes with the purpose of using them in the transformation of Colombian soybean (Glycine max) varieties to confer them tolerance to glyphosate. We transformed Nicotiana benthamiana as a model to validate the functionality of the expression cassettes and detected the expression of the transgene by RT-PCR. Additionally, a Freedom to Operate analysis of the sequences used in the expression cassettes suggests that their commercial use in Colombia does not infringe third party rights. This analysis must be updated and validated by intellectual property experts prior to commercialization.
dc.formatapplication/pdf
dc.identifier0120-9965
dc.identifierhttps://www.redalyc.org/articulo.oa?id=180376041001
dc.identifierhttps://www.redalyc.org/journal/1803/180376041001/
dc.identifierhttps://www.redalyc.org/journal/1803/180376041001/html/
dc.identifierhttps://www.redalyc.org/journal/1803/180376041001/180376041001.epub
dc.identifierhttps://www.redalyc.org/journal/1803/180376041001/movil
dc.identifier10.15446/agron.colomb.v38n2.79150
dc.identifier.urihttp://hdl.handle.net/123456789/421831
dc.languageen
dc.publisherUniversidad Nacional de Colombia
dc.relationhttp://www.redalyc.org/revista.oa?id=1803
dc.rightsAgronomía Colombiana
dc.sourceAgronomía Colombiana (Colombia) Num.2 Vol.38
dc.subjectAgrociencias
dc.titleFreedom to operate analysis, design and evaluation of expression cassettes that confer tolerance to glyphosate
dc.typeartículo científico

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Agronomía Colombiana - Universidad Nacional de Colombia