Karyotype characterization of Crotalaria juncea (L.) by chromosome banding and physical mapping of 18S-5.8S-26S and 5S rRNA gene sites.

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dc.contributorMATEUS MONDIN, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JANAY ALMEIDA DOS SANTOS-SEREJO, CNPMF; MARGARIDA L. R. AGUIAR-PERECIN, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ.
dc.creatorMONDIN, M.
dc.creatorSANTOS-SEREJO, J. A. dos
dc.creatorAGUIAR-PERECIN, M. L. R.
dc.date2026-06-03T18:59:08Z
dc.date2026-06-03T18:59:08Z
dc.date2008-02-11
dc.date2007
dc.date.accessioned2026-07-07T04:53:10Z
dc.descriptionThe chromosomes of Crotalaria juncea, a legume of agronomic interest with a 2n = 16 karyotype composed of metacentric chromosomes, were analyzed using several cytogenetic techniques. C-banding revealed heterochromatic regions around the centromeres in all chromosomes and adjacent to the secondary constriction on the chromosome 1 short arm. Fluorescent staining with the GC-specific chromomycin A3 (CMA) highlighted these heterochromatic regions and a tiny site on the chromosome 1 long arm while the AT-specific stain 4'-6-diamidino-2-phenylindole (DAPI) induced a reversed pattern. Staining with CMA combined with AT-specific distamycin A (DA) counterstaining quenched the pericentromeric regions of all chromosomes, but enhanced fluorescence was observed at the heterochromatic regions around the secondary constriction and on the long arms of chromosomes 1 and 4. Fluorescence in situ hybridization (FISH) revealed 18S-5.8S-26S rRNA gene sites (45S rDNA) on chromosomes 1 and 4, and one 5S rDNA locus on chromosome 1. All the rDNA sites were co-located with the positive-CMA/DA bands, suggesting they were very rich in GC. Silver staining revealed signals at the main 45S rDNA locus on chromosome 1 and, in some cells, chromosome 4 was labeled. Two small nucleoli were detected in a few interphase cells, suggesting that the minor site on chromosome 4 could be active at some stages of the cell cycle.
dc.identifierGenetics and Molecular Biology, Ribeirão Preto, v. 30, n. 1, p. 65-72, 2007.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/654622
dc.identifierhttps://doi.org/10.1590/S1415-47572007000100013
dc.identifier.urihttp://hdl.handle.net/123456789/474223
dc.languageeng
dc.rightsopenAccess
dc.subjectCrotalaria juncea karyotype
dc.subjectC-bands
dc.subjectFluorescent banding
dc.subjectrDNA sites
dc.subjectAg-NOR
dc.subjectCrotalária Juncea
dc.subjectLeguminosa
dc.subjectCariótipo
dc.subjectfish
dc.titleKaryotype characterization of Crotalaria juncea (L.) by chromosome banding and physical mapping of 18S-5.8S-26S and 5S rRNA gene sites.
dc.typeArtigo de periódico

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