Fish nutrition consultancy report,27 September to 5 December 1991
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The terms of reference were discussed with the Government Project Officer (GPC) on arrival and it was decided that the most pressing needs were to provide in service training for government scientists on the methods used to quantify the levels of lipids, amino acids and vitamin C in fish feeds and fish tissues. As mentioned in my 1990 FAO Final Report on the project, this was made more critical by the assignment in August, 1990, of Nanthiya Unprasert to Mississippi State University, where she is pursuing a Ph.D. in fish nutrition and will be occupied until 1993, at least. Until 1990, prior to my consultancy, Ms. Unprasert was the only government scientist in the Fish Nutrition Project at NIFI who had sufficient training to operate the High-Performance Liquid Chromatograph (HPLC) for analyzing amino acids and vitamins, or the Gas Chromatograph (GC) for analyzing fatty acid methyl esters. Therefore, the progress of research at NIFI was slowed after Ms. Unprasert's assignment to Mississip pi State University by the lack of trained staff to conduct the required analytical determinations needed to complement the planned feeding trials central to the mission of NIFI and the FAO/UNDP project. Training was therefore provided in 1990 to Dr. Wimol, Mr. Pairat Kosutarak, and Ms. Amornrat Sermwatanakul to establish familiarity with HPLC equipment, especially which elements of HPLC operation were most critical with regard to safety and equipment integrity. In 1991, Dr. Wimol was much more confident on HPLC operation, and no additional training on basic HPLC operation was required other than a brief review of procedures.