High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots

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dc.creatorBelalcázar, John Eiver
dc.creatorDufour, D.L.
dc.creatorAndersson, Meike S.
dc.creatorPizarro, Mónica
dc.creatorLuna, Jorge
dc.creatorLondoño Hernandez, Luis Fernando
dc.creatorMorante, Nelson
dc.creatorJaramillo Valencia, Angélica M.
dc.creatorPino, Lizbeth
dc.creatorBecerra López Lavelle, Luis Augusto
dc.creatorDavrieux, Fabrice
dc.creatorTalsma, Elise F.
dc.creatorCeballos, H.
dc.date2016-11
dc.date2016-10-13T15:24:24Z
dc.date2016-10-13T15:24:24Z
dc.date.accessioned2026-06-27T14:31:15Z
dc.descriptionPast research developed reliable equations to base selections for high β-carotene on near-infrared spectroscopy (NIR) predictions (100 genotypes d−1) rather than with high-performance liquid chromatography (HPLC) (<10 samples d−1). During recent harvest, CIAT made selections based on NIR predictions for the first time. This innovation produced valuable information that will help other cassava (Manihot esculenta Crantz) breeding programs. A total of 284 samples were analyzed with NIR and HPLC for total β-carotene (TBC) and by the oven method for dry matter content (DMC). Results indicated that NIR reliably predicted TBC and DMC. In addition, 232 genotypes grown in preliminary yield trials (PYTs) were harvested at 8.5 and 10.5 mo after planting (one plant per genotype and age) and root quality traits analyzed (by NIR only). Repeatability of results at the two ages was excellent, suggesting reliable results from NIR. In contrast to previous reports, age of the plant did not influence carotenoids content in the roots. The availability of a high-throughput NIR protocol allowed comparing results (for the first time) from seedling and cloned plants from the same genotype. Results showed very little relationship for DMC between seedling and cloned plants (R2 = 0.09). There was a much better association for TBC (R2 = 0.48) between seedling and cloned plants. It is postulated that variation in the environmental conditions when seedling and cloned plants (from the same genotype) may be responsible for these weak associations. Important changes in selection strategies have been implemented to overcome problems related to a lengthy harvesting season.
dc.identifierhttps://hdl.handle.net/10568/77289
dc.identifier.urihttp://hdl.handle.net/123456789/80568
dc.languageen
dc.publisherWiley
dc.rightsOpen Access
dc.sourceBelalcazar, John; Dufour, Dominique; Andersson, Meike S.; Pizarro, Mónica; Luna, Jorge; Londoño, Luis; Morante, Nelson; Jaramillo, Angélica M.; Pino, Lizbeth; Becerra López-Lavalle, Luis Augusto; Davrieux, Fabrice; Talsma, Elise F.; Ceballos, Hernán. 2016. High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots . Crop Science 56 (6): 2916-2928.
dc.subjectmanihot esculenta
dc.subjectplant breeding
dc.subjectcarotenoids
dc.subjectspectroscopy
dc.subjectgenotypes
dc.subjectfitomejoramiento
dc.subjectcarotenoides
dc.subjectespectroscopia
dc.subjectmarcadores genéticos
dc.titleHigh-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots
dc.typeJournal Article

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