Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat

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dc.creatorCarla Bertechini FARIA
dc.creatorGiovana Caputo ALMEIDA-FERREIRA
dc.creatorKarina Bertechine GAGLIARDI
dc.creatorTatiane Cristina Albuquerque ALVES
dc.creatorDauri José TESSMANN
dc.creatorMiguel MACHINSKI JUNIOR
dc.creatorIone Parra BARBOSA-TESSMANN
dc.date2012
dc.date.accessioned2026-07-07T04:18:56Z
dc.descriptionThe detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum . However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum .
dc.formatapplication/pdf
dc.identifier0101-2061
dc.identifierhttps://www.redalyc.org/articulo.oa?id=395940112030
dc.identifier.urihttp://hdl.handle.net/123456789/457550
dc.languageen
dc.publisherSociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.relationhttp://www.redalyc.org/revista.oa?id=3959
dc.rightsCiência e Tecnologia de Alimentos
dc.sourceCiência e Tecnologia de Alimentos (Brasil) Num.1 Vol.32
dc.subjectAgrociencias
dc.titleUse of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat
dc.typeartículo científico

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Ciência e Tecnologia de Alimentos - Sociedade Brasileira de Ciência e Tecnologia de Alimentos