Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt

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dc.creatorNakato, G.V.
dc.creatorAkinbade, S.A.
dc.creatorKumar, P. Lava
dc.creatorBandyopadhyay, Ranajit
dc.creatorBeed, Fenton D.
dc.date2013-12
dc.date2017-07-25T10:26:01Z
dc.date2017-07-25T10:26:01Z
dc.date.accessioned2026-06-27T16:09:13Z
dc.descriptionRabbit polyclonal antibodies (PAbs) were produced against a pure isolate of Xanthomonas campestris pv. musacearum (Xcm), which is the causal organism of banana Xanthomonas wilt (BXW) disease of banana (Musa spp.) in the Great Lakes region of Africa. Anti-Xcm PAbs were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) for the detection of Xcm in leaf and pseudostem tissues of infected banana and plantain (Musa spp.). The anti-Xcm PAbs in DAC-ELISA specifically reacted with Xcm but not with Escherichia coli, X. vasicola pv. vasculorum of maize and sugarcane or X. vasicola pv. holcicola of sorghum. Results of the DAC-ELISA detection of Xcm are comparable with the results of PCR and culture-based detection assays for the same pathogen.
dc.identifierhttps://hdl.handle.net/10568/82866
dc.identifier.urihttp://hdl.handle.net/123456789/123979
dc.languageen
dc.publisherCAB International
dc.rightsLimited Access
dc.sourceNakato, G.V., Akinbada, S.A., Kumar, P.L., Bandyopadhyay, R. & Beed, F. (2013). Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt. In G. Blomme, P. van Asten and B. Vanlauwe, Banana systems in the humid highlands of sub-Saharan Africa. (p. 93-100). Wallingford: CABI.
dc.subjectpolyclonal antibodies
dc.subjectbananas
dc.subjectdiseases
dc.subjectrabbit polyclonal antibodies
dc.subjectbanana xanthomonas wilt
dc.subjectxanthomonas campestris pv. musacearum
dc.titleDevelopment of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt
dc.typeBook Chapter

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Instituto Internacional de Agricultura Tropical (IITA)