Genome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.

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dc.contributorBrenda Neves Porto, Universidade Federal de Lavras - UFLA. Progama de Pós-graduação em Biotecnologia Vegetal
dc.contributorEVELINE TEIXEIRA CAIXETA MOURA, CNPCa
dc.contributorSandra Marisa Mathioni, Universidade Federal de Lavras - UFLA/Departamento de Fitopatologia
dc.contributorPedro Marcus Pereira Vidigal, Universidade Federal de Viçosa - UFV/Núcleo de Análises de Biomoléculas
dc.contributorLaércio Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia
dc.contributorEunize Maciel Zambolim, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia
dc.contributorNicole Donofrio, University of Delaware/Department of Plant and Soil Sciences
dc.contributorShawn W. Polson, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology
dc.contributorThiago Andrade Maia, Universidade Federal de Viçosa - UFV/Departamento de Fitopatologia
dc.contributorChuming Chen, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology
dc.contributorModupe Adetunji, Delaware Biotechnology Institute/Center for Bioinformatics and Computational Biology
dc.contributorBrewster Kingham, University of Delaware, Newark/Delaware Biotechnology Institute/Sequencing and Genotyping Center
dc.contributorRonaldo José Durigan Dalio, Instituto Agronoˆmico de Campinas - IAC/Centro de Citricultura “Sylvio Moreira”
dc.contributorMário Lúcio Vilela de Resende, Departamento de Fitopatologia, Universidade Federal de Lavras.
dc.creatorPORTO, B. N.
dc.creatorCAIXETA, E. T.
dc.creatorMATHIONI, S. M.
dc.creatorVIDIGAL, P. M. P.
dc.creatorZAMBOLIM, L.
dc.creatorZAMBOLIM, E. M.
dc.creatorDONOFRIO, N.
dc.creatorPOLSON, S. W.
dc.creatorMAIA, T. A.
dc.creatorCHEN, C.
dc.creatorADETUNJI, M.
dc.creatorKINGHAM, B.
dc.creatorDALIO, R. J. D.
dc.creatorRESENDE, M. L. V. de
dc.date2019-10-18T18:10:13Z
dc.date2019-10-18T18:10:13Z
dc.date2019-10-18
dc.date2019
dc.date2019-10-18T18:10:13Z
dc.date.accessioned2026-07-01T00:26:45Z
dc.descriptionCoffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases of coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of its secretome, we aimed to sequence and assemble the entire H. vastatrix genome using two next-generation sequencing platforms and a hybrid assembly strategy. This resulted in a 547 Mb genome of H. vastatrix race XXXIII (Hv33), with 13,364 predicted genes that encode 13,034 putative proteins with transcriptomic support. Based on this proteome, 615 proteins contain putative secretion peptides, and lack transmembrane domains. From this putative secretome, 111 proteins were identified as candidate effectors (EHv33) unique to H. vastatrix, and a subset consisting of 17 EHv33 genes was selected for a temporal gene expression analysis during infection. Five genes were significantly induced early during an incompatible interaction, indicating their potential role as pre-haustorial effectors possibly recognized by the resistant coffee genotype. Another nine genes were significantly induced after haustorium formation in the compatible interaction. Overall, we suggest that this fungus is able to selectively mount its survival strategy with effectors that depend on the host genotype involved in the infection process.
dc.identifierPLOSONE, April 18, 2019.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1113216
dc.identifier.urihttp://hdl.handle.net/123456789/404219
dc.languageeng
dc.rightsopenAccess
dc.subjectHemileia Vastatrix
dc.subjectFungo
dc.subjectFerrugem
dc.subjectGenome
dc.subjectLeaf rust
dc.subjectInfection
dc.titleGenome sequencing and transcript analysis of Hemileia vastatrix reveal expression dynamics of candidate effectors dependent on host compatibility.
dc.typeArtigo de periódico

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